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BIKAI 1511 Bio Biological Inert Liquid Chromatography System in use of application for small nucleic acid drug analysis
Release time:
2025-05-28
Small interfering RNA (siRNA) drugs, as the most promising therapeutic drugs currently available, have the characteristics of high specificity, wide target range, and can achieve long-lasting effects. SiRNA generally refers to double stranded RNA with a length of 19-23 nucleotide sequences, and chemical synthesis is the most direct and reliable method to obtain siRNA. The liquid chromatography methods commonly used for purity and impurity analysis include reverse phase ion pair chromatography and ion exchange chromatography. This article uses the BIKAI 1511 Bio bioinert system to rapidly separate the positive and negative sense chains and their impurities in siRNA drugs using anion exchange chromatography. The experimental results are reliable and consistent with the peak shape and results of a foreign brand of biological inertness instrument. The BIKAI 1511 Bio biological inert system meets the requirements of nucleic acid drugs for high-performance liquid chromatography inert systems, and can be applied to nucleic acid drug purity analysis, impurity analysis, etc.
Chromatographic conditions
System：BIKAI 1511 Bio - Biological inertness system
Column：LotusBio SAX（4.6×250 mm, 5 μm）
Flow rate：0.5 mL/min
Mobile phase：A：20 mM - buffer salt
B：A+1.5M NaCl
Injecion volume：5 μL (Sample concentration is about 2 mg/mL)
Column temperature：30℃
UV detection wavelength：260 nm
Figure 1 - SiRNA positive and negative sense chains and mixed spectra of impurity samples—BIKAI 1511 Bio
Table 1 - Results of siRNA positive and negative sense chains and impurity samples
Figure 2 - Mixed spectrum of siRNA positive and negative sense chains and impurity samples - a certain brand of biological inert instrument
Introduction to BIKAI 1511 Bio - Inert Liquid Chromatography System
BIKAI 1511 Bio - bio inert liquid chromatography system adopts a bio inert sample flow path, which is corrosion-resistant and anti adsorption, making it easily suitable for biological and extreme pH applications, minimizing losses caused by metal adsorption. Using biologically inert materials to reduce protein adsorption can better restore the true peak area of the sample and ensure accurate test results.
- Excellent biological inertness
- Sample flow path with biological inertness
- PEEK, Titanium and other biocompatible materials
- Easy to apply to biological and extreme pH applications
- Minimize losses caused by metal adsorption to the greatest extent possible
Higher Peak, More realistic area
- Higher experience sample delivery
- Contact type high-efficiency integrated cooling system, quickly reaching the set temperature range of 4 ℃ - room temperature
- Scalable to 4 x sample tray, capable of holding 216 bits (54 4) or 384 bits (96 4) for higher throughput
- High repeatability without sample loss
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